تولید فورسکولین و بیان ژن 1- دیاکسی-D-زایلولوز-5- فسفات ردوکتوایزومراز در گیاه حسنیوسف (Coleus forskohlii) تیمار شده با مس | ||
| دوفصلنامه فنآوری تولیدات گیاهی | ||
| مقاله 4، دوره 6، شماره 2، آذر 1393، صفحه 49-60 اصل مقاله (800.99 K) | ||
| نوع مقاله: مقاله علمی-پژوهشی | ||
| نویسندگان | ||
| عفت شهبازی1؛ علی ریاحی مدوار* 2 | ||
| 1دانشآموخته کارشناسی ارشد رشته بیوتکنولوژی کشاورزی، دانشگاه تحصیلات تکمیلی صنعتی و فناوری پیشرفته، کرمان، ایران. | ||
| 2گروه بیوتکنولوژی، پژوهشکده علوم محیطی، پژوهشگاه علوم و تکنولوژی پیشرفته و علوم محیطی، دانشگاه تحصیلات تکمیلی صنعتی و فناوری پیشرفته، کرمان، ایران | ||
| چکیده | ||
| فورسکولین، ماده مؤثره گیاه Coleus forskohlii است که خواص درمانی متعددی دارد. در این تحقیق، میزان تولید فورسکولین و همچنین بیان ژن آنزیم 1-دیاکسی-D- زایلولوز-5- فسفات ردوکتوایزومراز (DXR) در گیاه C. forskohlii تیمار شده با غلظتهای مختلف (صفر (بهعنوان گیاه شاهد)، 4، 8 و 16 میکرومولار) الیسیتور مس بهمدت 16 ساعت بررسی گردید. نتایج نشاندهنده افزایش تولید فورسکولین تا غلظت 8 میکرومولار است، بهطوریکه در این تیمار، مقدار فورسکولین حدود 5/1 برابر مقدار آن در نمونه شاهد افزایش یافت که با تغییرات سطح بیان ژن DXR همخوانی داشت. ثابت شده است که حضور اضافی مس باعث تولید مولکولهای فعال اکسیژن میشود که بهطور مستقیم و یا با واسطه مولکولهای سیگنالینگ دیگر، بیان ژنهای درگیر در سیستم دفاعی گیاه را فعال میکنند. با توجه به نقش دفاعی دیترپنها، احتمال داده میشود نتیجه افزایش بیان ژن DXR تحت تیمار با این الیسیتور، افزایش تولید فورسکولین است که نقش محافظتی در برابر تنشهای را داراست. | ||
| کلیدواژهها | ||
| الیسیتور؛ بیان ژن؛ کاروتنوئید؛ کلروفیل | ||
| موضوعات | ||
| اصلاح و فیزیولوژی گیاهان دارویی؛ بیوتکنولوژی | ||
| عنوان مقاله [English] | ||
| Forskolin Production and Gene Expression of 1-Deoxy-D-Xylulose-5-Phosphate Reductoisomerase in Treated Coleus forskohlii Plant with Cu | ||
| نویسندگان [English] | ||
| Ali Riahi-Madvar2؛ | ||
| چکیده [English] | ||
| Forskolin is an effective compound found in Coleus forskohlii plant which has different therapeutic properties. In this research, production of forskolin and gene expression of 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR) were investigated in C. forskohliiplant after treatment with different concentrations of copper elicitor (0; as control, 4, 8 and 16 µM) for 16 hours. Results showed that forskolin concentration is increased up to 8 µM in such a way that the concentration can be estimated 1.5 fold more than the control sample, which consisted with changes of DXR gene expression level. It has been proved that excess concentration of copper, causes triggering of reactive oxygen species that directly or mediated through others signaling molecules induce gene expression level of defensive system. Considering the defensive role of diterpens, it is proposed that increase in DXR gene expression level by copper elicitor leads to increase in forskolin concentration as protective compound against stresses. | ||
| کلیدواژهها [English] | ||
| Elicitor, Gene expression, Carotenoid, Chlorophyll | ||
| مراجع | ||
|
Adriano, D. C. 2001. Trace elements in terrestrial environments; Biochemistry, Bioavailability and Risk of Metals, Springer-Verlag, New York.
Agarwal, A. G. and Parks, R. E. 1983. Forskolin: a potential antimetastatic agent. International Journal of Cancer, 32: 801-804.
Al-Bader, M. D. 2006. Estrogen receptors alpha and beta in rat placenta: detection by RT-PCR, real time PCR and western blotting. Reproductive Biology and Endocrinology, 28: 4-13.
Ammon, H. P. T. and Muller, A. B. 1985. Forskolin: from an ayurvedic remedy to a modern agent. Planta Medica 46: 473-477.
Bailey, L. H. 1942. Standard Cyclopedia of Horticulture, Macmillan, New York.
Bouvier, F., Rahier, A. and Camara, B. Biogenesis, 2006. Molecular regulation and function of plan Isoprenoids, Institut de Biologie Mole´culaire des Plantes du CNRS.
Bustin, S. A. and Dorudi, S. 1998. Molecular assessment of tumour stage and disease recurrence using PCR-based assays, Molecular MedicineToday, 4: 389-396.
Cantrill, R. 2008. Reviewed by Yoko Kawamura, Phytosterols, phytostanols and their esters, Chemical and Technical Assessment.
Caprioli, J., Sears, M., Bausher, L., Gregory, D. and Mead, A. 1984. Forskolin lowers intraocular pressure by reducing aqueous inflow. Investigative Ophthalmology and Visual Science, 25: 268-277.
Schaneberg, B.T. and Khan, I.A. 2003. Quantitative analysis of forskolin in Coleus forskohlii (Lamiaceae) by reversed-phase liquid chromatography. Journal of AOAC International, 86(3) :467-70.
De Souza, N. J. and Shah, V. 1988. Forskolin an adenylate cyclase activating drug from an Indian herb. Economic and Medicinal Plant Research 2: 1-16.
Dubey, M. P., Srimal, R. C., Patnaik, G. K. and Dhawan, B. N. 1974. Blood pressure lowering and antispasmodic effects of C. forskohlii Briq. Indian Journal of Pharmaceutical Science, 6:15.
Dubey, M.P., Srimal, R. C., Nityanand, S. and Dhawan, B. N. 1981. Pharmacological studies on Coleonol, a hypotensive diterpene from Coleus forskohlii. Journal of Ethnopharmacology, 3: 1-13.
Engprasert, S., Taura, F., Kawamukai, M. and Shoyama, Y. 2004. Molecular cloning and functional expression of geranylgeranyl pyrophosphate synthase from Coleus forskohlii Briq, BMC Plant Biology, 18:18.
Haye, B., Aublin, J. L. and Champion, S. 1985. Chronic and acute effects of forskolin on isolated thyroid cell metabolism. Molecular and Cellular Endocrinology, 43: 41-50.
Hirschberg, J. 2001. Carotenoid biosynthesis in flowering plants, Current Opinion in Plant Biology, 4: 210-218.
Laule, O., Fürholz, A., Chang, H.S., Zhu, T., Wang, X., Heifetz, P. B., Gruissem, W., and Lange, B. M. 2003. Crosstalk between cytosolic and plastidial pathways of isoprenoid biosynthesis in Arabidopsis thaliana. Proceedings of the National Academy of Sciences of the USA, 100: 6866-6871.
Lichtenthaler, H. K., Schwender, J., Disch, A. and Rohmer, M. 2002. Biosynthesis of isoprenoids in higher plant chloroplasts procceds via a Mevalonate-independent pathway. Planta, 216: 271-274.
Marone, V., Columbo, M., Triggiani, M., Cirillo, R., Genovese, A. and Formisano, S. 1987. Inhibition of IgEmediated release of histamine and peptide leukotriene from human basophils and mast cells by forskolin. Biochemical Pharmacology, 36: 13-20.
Misr, L. N., Tyagi, B. R., Ahmad, A. and Bahl, J,R. 1994. Variablity in the chemical composition of the essential oil of Coleus forskohlii genotypes. Journal of Essential Oil Research, 6: 243-247.
Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiology Plant, 15: 473-497.
Place, R. F., Li L. C., Pookot, D., Noonan, E. J. and Dahiya, R. 2008. MicroRNA-373 induces expression of genes with complementary promoter sequences. Proceedings of the National Academy of Sciences USA, 105: 8-13.
Radykewicz, T., Rohdich, F., Wungsinthaweekul, J., Herz, S., Kis, K., Eisenreich, W., Bacher, Zenk, M. H. and Arigoni, D. 2000. Biosynthesis of terpenoids:1-deoxy-D-xylulose-5-phosphate reductoisomerase from Escherichia coli is a class B dehydrogenase, Febs Letters, 465: 157-160.
Rezaee, F, Ghanati, F. and Behmanesh, M. 2013. Antioxidant activity and expression of catalase gene of Eustoma grandiflorum L. in response to boron and aluminum. South African Journal of Botany, 84: 13-18.
Roger, P. P., Servais, P. and Dumont, J. E. 1987. Regulation of dog thyroid epithelial cell cycle by forskolin and adenylate cyclase activator. Experimental Cell Research, 172: 282-292.
Rohmer, M. 2003. Mevalonate-independent methylerythritol phosphate pathway for isoprenoid biosynthesis, Elucidation and distribution. Pure and Applied Chemistry, 75: 375-387.
Seamon, K. B. 1984. Forskolin and adenylate cyclase: New opportunities in drug design. In: Annual Report on Medical Chemistry. (D. M., Bailey eds.). Academic Press, New York, 19: 293-302.
Shah, V. Bhat, S. V. Bajwa, B. S. Dornaeur, H. and De Souza, N. N. J. 1980. The occurrence of forskolin in Labiatae. Planta Medica, 39: 183-185.
Shan, Y., Xu, L., Lu, Y., Wang, X., Zheng, Q., Kong, L. and Niwa, M. 2008. Diterpenes from Coleus forskohlii. Chemical and Pharmaceutical Bulletin, 56: 52-56.
Walker, C.D. and Webl, J., 1981. Copper in plants. Form and behaviours, in: Loner-agan J. F., Robson A.D. and Graham R. D., (Eds.), Copper in soils and plants. Academic Press, London, 189-212.
Wong, S., Mok, W., Phaneuf, S., Katz, S. and Salari, H. 1993. Forskolin inhibits platelet-activating factor binding to platelet receptors independently of adenylyl cyclase activation. European Journal of Pharmacology, 245: 55-61.
Yruela, I. 2009. Copper in plants: acquisition, transport and interactions. Functional Plant Biology, 36: 409-430.
Zhou, M. L., Zhu, X. M., Shao, J. R., Wu, Y. M. and Tang, Y. X. 2010. Transcriptional response of the catharanthine biosynthesis pathway to methyl jasmonate/nitric oxide elicitation in Catharanthus roseus hairy root culture. Applied Microbiology and Biotechnology, 88: 737-750.
| ||
|
آمار تعداد مشاهده مقاله: 3,301 تعداد دریافت فایل اصل مقاله: 1,308 |
||